Live imaging of Dorsal-Venus with two-photon light-sheet microscopy
The Dorsal-Venus signal from dl1/+; dl-venus/H2A-RFP mothers is presented on the left. The embryo was imaged at 150 μm from anterior pole starting at nc 11 until the beginning of gastrulation. The accompanying plot on the right displays analysis corresponding to each time point of the movie. Intensity level of each nucleus at the given location along the DV axis is represented by green dots, and the quantified Dorsal gradient is shown by the red curve.