Live Imaging of Dorsal-Venus with Two-Photon Light-Sheet Microscopy

The Dorsal-Venus signal from dl1/+; dl-venus/H2A-RFP mothers is presented on the left. The embryo was imaged at 150 μm from anterior pole starting at nc 11 until the beginning of gastrulation. The accompanying plot on the right displays analysis corresponding to each time point of the movie. Intensity level of each nucleus at the given location along the DV axis is represented by green dots, and the quantified Dorsal gradient is shown by the red curve.

*Reeves GT, *Trisnadi N, Truong TV, Nahmad M, Katz S, and Stathopoulos A. Dorsal-Ventral Gene Expression in the Drosophila Embryo Reflects the Dynamics and Precision of the Dorsal Nuclear Gradient. Dev Cell. 22(3)544-557. Epub 14 Feb 2012.

CVM cell migration from dorsal view in different genetic backgrounds

Compilation of five movies of embryos of five different genetic backgrounds (wild type, htlAB42, DfBSC25, pyr and ths mutants) in which CVM cell migration was imaged live at embryonic stage 10, 11 and 12 using the bHLH54F-H2A-mCherry reporter. Anterior is towards the left and dorsal view is in focus.

Kadam S, Ghosh S, and Stathopoulos A. Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands. Development. 139(4). Epub 4 Jan 2012.

Segmentation and tracking of mesoderm nuclei in wild-type embryos using Imaris software

Spots represent mesoderm nuclei tracked over time. Ectoderm H2A-GFP raw data is kept in for reference. The first view is dorsal, and the second view is posterior. Large grid marks = 20 µm.

*McMahon A, *Supatto W, Fraser SE, Stathopoulos A. Dynamic Analyses of Drosophila Gastrulation Provide Insights into Collective Cell Migration. Science. 322(5907):1546-1550. 5 Dec 2008.

©2014 Stathopoulos Lab, Caltech Division of Biology & Biological Engineering.         
Design by N Trisnadi. Last updated 18 Feb 2014.